Dr. Talluri Thirumala Rao

Dr. Talluri Thirumala Rao
Sr. Scientist
Qualifications : MVSc., PhD
Field of Specialization : Animal Reproduction and Gynaecology, Reproductive Biotechnology

Research Interests My area of research includes Stem cell Biology, Reproductive Biotechnology, Assisted Reproductive Technologies and Transgenic Animal production through sperm mediated gene transfer. Presently working on the projects aiming at the elucidation of fertility markers affecting the stallion fertility.
Recent research papers in last five years:

  • Talluri TR, Kumar D, Glage S, Garrels W, Ivics Z, Debowski K, Behr R, Kues WA. 2014. Non-viral reprogramming of fibroblasts into induced pluripotent stem cells by Sleeping Beauty and piggyBac transposons. Biochemical and Biophysical Research Communications 450, 581-587.
  • Thirumala R. Talluri, Dharmendra Kumar, Silke Glage, WiebkeGarrels, Zoltan Ivics, Katharina Debowski, Rudiger Behr, Heiner Niemann, and Wilfried A. Kues. (2015).Derivation and Characterization of Bovine Induced Pluripotent Stem Cells by Transposon-Mediated Reprogramming. CELLULAR REPROGRAMMING (Volume 17, Number 2, 2015), DOI: 10.1089/cell.2014.0080 (1.462)
  • Kues WA., Talluri TR., Garrels W. 2013. Delineating the placental maternal-fetalinterface. Molecular Reproduction and Development 80, 959.
  • Arangasamy A, Thirumala Rao Talluri, S. K. Ravi, R. K Singh and Yash Pal 2013. Freezing of Indigenous Stallion and Poitou Jack Semen with Amide as Cryoprotectants. Veterinary Practitioner Vol. 14 No. 2 (Supple. 1), P 469-470.
  • S.K. Ravi, P.S. Yadav, R.K. Singh, Thirumala Rao Talluri, Y. Pal, A. Arangasamy, J Singh, R.C. Sharma and SusaviKumari, 2013. An Overview on Reproductive Disorders in Indigenous Female Equids. Journal of Animal Research: Vol. 3(2) December 2013, P 245-259.
  • S K Ravi, R A Legha, Yash Pal and Thirumala Rao Talluri (2013). Effect of different levels of glycerol on freezability of jack Semen. Veterinary Practitioner, 14 (2):338-339.(3.78)
  • Garrels W, Mukherjee A, Holler S, Cleve N, Talluri TR, Barg-Kues B, Diederich M, Köhler P, Petersen B, Lucas-Hahn A, Niemann H, IzsvákZ,Ivics Z, Kues WA. Identification and re-addressing of a transcriptionally permissive locus in the porcine genome. . Transgenic Res. 2015 Oct 29.
  • Arangasamy, Thirumala Rao Talluri, R. K Singh, Yash Pal and S.N. Tandon. 2015. Unilateral cryptorchidism in a Marwari stallion. Vet Practitioner. 2015 Vol.16 No.1 pp.132-133 ref.10
  • Thirumala Rao Talluri, SK Ravi, J Singh, RA Legha, Yash Pal. 2015. Application of Caslick’s Index in Predicting the Mare Fertility. Journal of Animal Research: v.5 n.2, p. 219-222. June 2015
  • Ana P. Alessio , Alejandro E. Fili, , Wiebke Garrels , Diego O. Forcato , María F. Olmos Nicotra , Ana C. Liaudat , Romina J. Bevacqua , Virginia Savy , María Inés Hiriart , Thirumala R. Talluri, Jesse B. Owens, Zoltan Ivics, Daniel F. Salamone, Stefan Moisyadi, Wilfried A. Kues and Pablo Bosch. 2015. THERIOGENOLOGY. 2015 Dec 29. pii: S0093-691X(15)00691-3. doi: 10.1016/j.theriogenology.2015.12.016 (1.838)
  • Kumar, D., Talluri, T.R., Anand, T., and Kues, W.A. (2015). Induced pluripotent stem cells: Mechanisms, achievements and perspectives in farm animals. World Journal of Stem Cells (26, 315-328).
  • Dharmendra Kumar, Thirumala R. Talluri, Taruna Anand, Wilfried A. Kues. 2015. Transposon-based Reprogramming to Induced Pluripotency. Histol. Histopathol. 2015 Aug 24:11656
  • Bosch P, Forcato DO, Alustiza FE, Alessio AP, Fili AE, Olmos Nicotra MF, Liaudat AC, Rodríguez N, Talluri TR, Kues WA. Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals. Cellular Molecular Life Sciences. DOI 10.1007/s00018-015-1842-1
  • S.K. Ravi, T.R. Talluri, R.A. Legha, A. Arangasamy, Y. Pal, J. Singh and P.S. Yadav (2015). Freezability and Fertility of Marwari Horse Semen at Farmer’s Doorstep. Livestock Research International, January-March, Vol 3, Issue 1: Pages 01-06.
  • Garrels W*, Talluri TR*, Apfelbaum R, Carratalá YP, Bosch P, Pötzsch K, Grueso E, Ivics Z, Kues WA.2016. One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle. SCI REP. NATURE .COM .2016 Feb 24;6:21953. doi: 10.1038/srep21953. (* These authors contributed equally to this work.)
  • Ayan Mukherjee, Wiebke Garrels, Thirumala R. Talluri, Daniela Tiedemann, Zsuzsanna Bösze Zoltan Ivics, Wilfried A. Kues. 2016. Expression of Active Fluorophore Proteins in the Milk of Transgenic Pigs Bypassing the Secretory Pathway. Scientific Reports, 6. Article number: 24464  (2016). Scientific Reports 6:24464 · April 2016 DOI: 10.1038/srep24464. www.nature.com/scientific reports.
  • Garrels W*, Talluri TR*, Ziegler M, Most I, Forcato DO, Schmeer M, Schleef M, Ivics Z, Kues WA. Cytoplasmic injection of murine zygotes with Sleeping Beauty transposon plasmids and minicircles results in the efficient generation of germline transgenic mice. BIOTECHNOL J. 2016 Jan;11(1):178-84. doi: 10.1002/biot.201500218. Epub 2015 Dec 4. (* These authors contributed equally to this work.)
  • Anand T*, Talluri TR*, Kumar D, Garrels W, Mukherjee A, Debowski K, et al. (2016) Differentiation of Induced Pluripotent Stem Cells to Lentoid Bodies Expressing a Lens Cell-Specific Fluorescent Reporter. PLoS ONE 11(6): e0157570. doi:10.1371/ journal.pone.0157570 ((* These authors contributed equally to this work.))
  • Rao Talluri T, Arangasamy A, Singh J, Ravi SK, Pal Y, Legha RA, Alpha Raj M, Chopra A, Singh RK, Tripathi BN, Factors affecting the Length of Gestation in Artificially Inseminated Marwari Mares of India, Asian Pacific Journal of Reproduction (2016), doi: 10.1016/j.apjr.2016.10.004.
  • R.A. Legha, P.A. Bala1, Yashpal, R.K. Dedar, V. Kumar, S.K. Ravi, T. Rao and A.K. Gupta (2016). Nutrients intake and energy requirement of Marwari mares at maintenance. Veterinary Practitioner Vol. 17 No. 1: 74-76
  • Thirumala Rao Talluri, S.K. Ravi, J. Singh, R.A. Legha, Yash Pal, A.K. Gupta, R.K. Singh and B.N. Tripathi. Feb 2016. Some reproductive parameters of Zanskari Mares reared in an organized farm under tropical climate. Indian Journal of Animal Sciences 86 (2): 163–167, February 2016/Article (6.00)
  • Arangasamy A, Thirumala Rao Talluri, Ravi S.K. Yash Pal: Synchronization of marwari mares for induction of estrus using prostaglandin F2 alpha. Indian Veterinary Journal, January 2016. (6.33)
  • Tejpal, J S Mehta, S K Ravi, T R Talluri, Ashok Kumar, Amit Kumar and Yogesh Soni (2016). Cryosurvival of Marwari stallion sperm in different extenders. Indian Journal of Animal Sciences 86 (12): 1396–1400, December 2016/Article
  • Talluri TR, Mal G, Ravi SK (2017) Biochemical components of seminal plasma and their correlation to the fresh seminal characteristics in Marwari stallions and Poitou jacks, Veterinary World, 10(2): 214-220.
  • Yogesh Soni, Thirumala Rao Talluri*, S. K. Ravi, Ashok Kumar Chaudhary, J. S. Mehta, R. A. Legha, Yash Pal, A.K.Gupta and B.N.Tripathi (2017). Quantitative and Qualitative assessment of seminal parameters of Manipuri breed stallions reared in arid zone of Rajasthan. Indian Journal of Animal Sciences 87 (5): 587–589, May 2017
  • Tejpal, J.S. Mehta, S.K. Ravi, Swati Ruhil, T.R. Talluri, Ashok Kumar and Diler Singh (2017) Study of certain physical parameters of fresh semen in Marwari horse. Veterinary Practitioner Vol. 18 No. 1 pg 47-51.
  • Yogesh Soni, Ashok Kumar, J. S. Mehta, T.R. Talluri, S. K. Ravi, R.A. Legha, B. N. Tripathi and Tejpal. Comparative study of fresh and frozen thawed seminal characteristics of three indigenous breeds of stallions. Veterinary Practitioner, Vol 18 (2) No 2; 252-256.
  • Tejpal, Ajay Kumar Ola, Diler Singh, Ashutosh Tripathi, Thirumala Rao Talluri and Ashok Kumar Chaudhary (2017). Dystocia in a buffalo due to hydrocephalic fetus: A case report. Buffalo Bulletin (October-December 2017) Vol.36 No.4.695-698.
  • Thirumala Rao Talluri, S K Ravi, Legha R A, Yash Pal, Thomas K Thomas, Sandeep Singh Kashyap and B N Tripathi (2018) Assessment of seminal traits and suitability of semen for cryopreservation of exotic stallions reared under Indian conditions. Indian Journal of Animal Reproduction 39 (1): June 2018.
  • T.R. Talluri, S.K. Ravi, C. Singh, R.A. Legha, S.C. Mehta, V. Prakash And B.N. Tripathi. 2019. Effect of two different permeable cryoprotectants on freezability of exotic stallion and jack semen. 27-30. Indian Journal of Animal Reproduction. 40:1 June 2019
  • Y. Soni, J.S. Mehta, A. Kumar, A. Tripathi and T.R. Talluri. 2019. Management of dystocia due to macerated fetus in a mare. Indian Journal of Animal Reproduction. 71-70, 40:1 June 2019.
  • Vijay Kumar, RA Legha, Yash Pal, RK Dedar, PA Bala, SK Ravi, TR Talluri, BN Tripathi. Physiological, hematological and biochemical responses in non-descript Indian donkeys working with different pack loads in brick kiln simulated conditions. International Journal Livestock Research, Vol 8 (06) Jun ’18.
  • NS Rathore, Kashyap S.K, Anupama Deora, Pankaj Kumar, J Singh, B N Tripathi, T R Talluri. 2018. Comparative study of different protocols for isolation of putative mesenchymal stem cells from equine umbilical cord wharton’s jelly. Indian Journal of Animal Sciences 88 (9): 1025–1029.
  • Yogesh Soni, T.R. Talluri∗, Ashok Kumar, S.K. Ravi, J.S. Mehta, B.N. Tripathi. 2018. Effects of different concentration and combinations of cryoprotectants on sperm quality, functional integrity in three Indian horse breeds. https://doi.org/10.1016/j.cryobiol.2018.12.005.

Technologies developed/ patented:


  • Stallion Semen cryopreservation at farmers’ door step
  • Mesenchymal stem cell (MSC) isolation protocols from Wharton’s jelly and Placenta of mare
  • Isolation and culture of MSC from fetal adnexa
  • Mare breeding sound examination using caslick’s Index
  • Development of alternative cryoprotectant to cryopreserve the stallion semen
  • Early detection of pregnancy through ultrasonography


Awards/ Fellowships:

  • ICAR-International Fellowship
  • Prof. S. S. Guraya Young Scientist Award from Indian Society for Society Reproduction and Fertility (ISSRF)
  • Society for Biology of Reproduction (TBR) Prize for the Abstract presentation at 2nd Joint German-Polish Conference on Reproductive Medicine; 46th Annual Conference of Physiology and Pathology of Reproduction; 38th Joint Conference on Veterinary and Human Reproductive Medicine in Gdansk, Poland, held from 27th Feb-1st March.
  • Reviewer Excellence award from Journal of Animal Research and Journal of Diary and food research for the years 2016 and 2018.



  • Life member of ISSAR (Indian Society for study of Animal Reproduction)
  • Life member of ISSRF (Indian society for Study for Reproduction and Fertility)
  • Life member of SAPI (Society for Animal Physiologists of India)


Students guided (Master’s & PhD) :
As a co Advisor/Research Guide


MVSc., : 2

PhD : 2


Ongoing Research Projects:
Principal Investigator (PI)


  • Assessment, evaluation and identification of physical, biochemical and genetic factors affecting stallion fertility
  • Approaches to the diagnosis and management of reproductive failure in equines



  • Endurance and fertility analysis in indigenous horses using single nucleotide polymorphisms markers
  • Assessment and optimization of equine management in an intensive system
    isolation culture and characterization of camel fibroblast cells and their cryopreservation
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