Dr. Anuradha Bhardwaj

Dr. Anuradha Bhardwaj
Senior Scientist
Qualifications : M.Sc. (Biotechnology), Ph.D. (Animal Biotechnology), PGDTMA

Field of Specialization: Donkey Milk, Reproductive Biotechnology, Genomics, Marker Assisted Selection and Population Genetics, Bioinformatics and Computational Biology

ResearchInterests: I am working in the area of equine genomics, marker-assisted selection and parentage verification, population genetics in Indian horses and bovines. My expertise includes those in reproductive biotechnology, recombinant DNA technology, recombinant protein production, proteomics, bioinformatics and computational biology and in silico pharmacology. My recent works have helped in identification, characterization, conservation and documentation of indigenous horses, donkeys and other equine breeds. My noteworthy works for recombinant proteins include those of cattle recombinant inhibin alpha and equine chorionic gonadotropins. My research also includes studies on characterization and applications of Donkey milk and development of Agri- entrepreneurship.

Recent research papers in last five years

  1. Bhardwaj, A Kumar, S., Nayan, V., Sharma, P., Pal, Y. Yadav, S.C.(2018) Expression and characterization of recombinant single chain beta-alpha equine chorionic gonadotropin in a prokaryotic host. Indian Journal of Animal Research, Article ID: B-3571; Online Published: 26-04-2018, (NAAS: 6.15), Publisher: ARCC, India.
  2. Gupta AK, Sanjay Kumar , Yash Pal , Anuradha Bhardwaj , Mamta Chauhan , Birendra Kumar , Prince and Vijh RK (2018). Genetic Diversity and Structure Analysis of Donkey Population Clusters in Different Indian Agro-climatic Regions. J Biodivers Endanger Species 2018, 6:S2. DOI: 10.4172/2332-2543.S2225.
  3. Bhardwaj, Nayan, Sharma, P., Kumar, S., Pal, Y. Singh, J. (2017). Molecular characterization, modeling, in silico analysis of equine pituitary gonadotropin alpha subunit and docking interaction studies with ganirelix. In Silico Pharmacology. 5(1):5. https://doi.org/10.1007/s40203-017-0025-1. (¶Co-first author: authors AB and VN contributed equally to this work), Publisher: Springer Berlin Heidelberg.
  4. Ashok Gupta; Sanjay Kumar, Parvati Sharma, Yash Pal, Ramesh Dedar, Jitender Singh, Anuradha Bhardwaj, Manoj Brahmane, Ajay Raut, Suresh Yadav, Birendra Singh (2016) “Biochemical profiles of Indian donkey population located in six different agro climatic zones” Comparative Clinical Pathology. (2016) 25:631–637. (doi:10.1007/s00580-016-2242-0)
  5. A.K. Gupta, Mamta Chauhan, Anuradha Bhardwaj and R. K. Vijh (2015). Assessment of Demographic Bottleneck in Indian Horse and Endangered Pony Breeds. Journal of Genetics. 94, e56–e62. (http://www.ias.ac.in/jgenet/OnlineResources/94/e56.pdf).
  6. A.K. Gupta, Mamta Chauhan, Anuradha Bhardwaj, Neelam Gupta, S.C. Gupta, Yash Pal, S.N. Tandon and R.K.Vijh (2014). Comparative Genetic Diversity Analysis among Six Indian Horse Breeds and English Thoroughbred. Livestock Science 163 (2014)1-11.
  7. Mamta Chauhan, A. K. Gupta, Yashpal Sharma, Anuradha Bhardwaj, Parvati Sharma. (2012). Efficacy of nine microsatellite markers in parentage testing of horse breeds. Indian Veterinary Journal, December 2014, 91 (12): 61 – 63.
  8. Varij Nayan, Anuradha Bhardwaj and Dheer Singh (2013). PAPP-A in Indian Water Buffalo (Bubalis bubalis) Ovary: Phylogeny, Expression, Hormonal Regulation and Sequence Characterization. Agricultural Research, 2(2):153–165.
  9. A.K. Gupta, Mamta Chauhan and Anuradha Bhardwaj (2013). Genetic diversity and bottleneck studies in endangered Bhutia and Manipuri pony breeds. Molecular Biology Reports, December 2013, Volume 30, Issue 12, pp 6935-6943.
  10. Anuradha Bhardwaj, Varij Nayan, S.De and S.L. Goswami (2013). Differential Expression Profiling of Recombinant Bovine Inhibin-Alpha at Reduced Temperature. Indian Journal of Animal Research, 47 (1): 61–65.
  11. Anuradha Bhardwaj, Varij Nayan, Paras Yadav, Sachinandan De, Tirtha K. Datta Surender Lal Goswami (2012). Heterologous Expression and Characterization of Indian Sahiwal Cattle (Bos indicus) Alpha Inhibin. Animal Biotechnology, 23 (2): 71-88.
  12. Gupta, A.K. Mamta Chauhan, Anuradha Bhardwaj and S.N. Tandon (2012). Microsatellite markers based genetic diversity and bottleneck studies in Zanskari pony. Gene, 499: 357-361.
  13. Gupta, A.K., Tandon, S.N., Pal, Y., Bhardwaj, A., Chauhan, M. (2012). Phenotypic characterization of Indian horse breeds – A comparative study. Animal Genetic Resources, 50, 49-58.
  14. Yash Pal, R A Legha, Niranjan Lal, Anuradha Bhardwaj, Mamta Chauhan, Sanjay Kumar, R C Sharma and A K Gupta (2012). Management and phenotypic characterization of donkeys of Rajasthan. Indian Journal of Animal Sciences, Volume 83, Issue 8, pp 793-797
  15. Anuradha Bhardwaj, Yash Pal, Ram Avtar Legha, Parvati Sharma, Varij Nayan, Sanjay Kumar, Hema Tripathi and B N Tripathi. 2020. Donkey milk composition and its therapeutic applications. Indian Journal of Animal Sciences. 90 (6): 837–841.

  16. Anuradha Bhardwaj, Nayan, V., Kumar, S., Sharma, P., Kumar, S., Chakarvarty, N., Kumar, S., Pal, Y., Yadav, S.C., Mohanty, A.K. & Tripathi, B.N. (2020). Expression Analysis of Recombinant Equine Chorionic Gonadotropin in Three Host Systems: E. coli BL21C, Sf insect cell lysate and COS-1 mammalian cells. Indian Journal of Animal Research, DOI: 10.18805/ijar.B-3917. (NAAS: 6.44), Publisher: ARCC, India.

  17. Yash Pal, Anuradha Bhardwaj, Ram Avtar Legha, Thirumala Rao Talluri, Sharat Chandra Mehta, Bhupendra Nath Tripathi. 2020. Indian Journal of Animal Research. Online forst. DOI:10.18805/IJAR.B-4221  | Article Id:B-4221.

  18. Bhatia, T., Nayan, V., Singh, R., Singh, C., Anuradha Bhardwaj, Kumar, S., Swaroop, M.N., Onteru, S.K., Sharma, R.K., Bharadwaj, A., Singh, D. and Mohanty, A.K., An Alternative Buffalo Urine-based Non-invasive Early Estrus Test using Wheat and Mung Bean Seed Germination. (2020, accepted). Indian Journal of Animal Research. Online first. Publisher: ARCC, India.

  19. Lyngdoh, E. L., Nayan, V., Vashisht, M., Kumari, S., Anuradha Bhardwaj, Bhatia, T., … & Singh, D. (2020). Gold nanoparticles modulate the steroidogenic and apoptotic pathway in a buffalo granulosa cell model. Biotechnology Letters, 42:1383–1395.

  20. Anuradha Bhardwaj, Kumari, P., Nayan, V., Legha, R. A., Gautam, U., Pal, Y., … & Tripathi, B. N. (2019). Estimation of Antioxidant Potential of Indigenous Halari and French Poitu Donkey Milk by using the Total Antioxidant Capacity and Ferric Reducing Antioxidant Power Essay. Asian Journal of Dairy and Food Research38(4), 307-310. (NAAS: 4.20).

  21. Anuradha Bhardwaj, Kumar, S., Nayan, V., Sharma, P., Pal, Y. & Yadav, S.C. (2019). Expression and characterization of recombinant single chain beta-alpha equine chorionic gonadotropin in prokaryotic host. Indian Journal of Animal Research, 53:587-593 (NAAS: 6.44), Publisher: ARCC, India.

Technologies developed/ patented: Parentage identification in equines (as a team member)

Awards/ Fellowships:

Received prestigious Women Scientist Award of the Society for Bioinformatics and Biological Sciences (SBBS) during International conference on ‘Recent trends in bioinformatics and biotechnology for sustainable development’ at SKUAST, Jammu-India in October 2017.

Best Oral Presentation Award in the area of proteomics and metabolomics during SVBBI National conference held at LUVAS, Hisar, Haryana in November 2018

Reviewers Excellence Award-2018 from Indian Journal of Animal Research (Agricultural Research Communication Centre-India).

Reviewer for the Journals: American Journal of Applied Sciences, Defence Life Science Journal, Journal of Primary Health Care General Practice, Indian Journal of Animal Sciences, Indian Journal of Animal Research, Biosciences, Biotechnology Research Asia journal.

ICAR-NAIP Research Fellowship (2013)


Life Member Society of Biological Chemists, India: 2005-present

Life Member Indian Society for Buffalo Development(ISBD):2010-present

SOCDAB: 2014-present

Life Member Indian Society for Veterinary Immunology and Biotechnology (ISVIB): 2015-present

Life Member Society for Bioinformatics and Biological Sciences (SBBS), India: 2017-present

International Society for Development and Sustainability (ISDS), Japan: 2017-present

The Organization for Women in Science for the Developing World: 2018-present

Students guided (Master’s): 1 (ongoing)
Ongoing Research Projects:
Research Projects

  • 1. Principal Investigator:” Studies on bioactive components and applications of donkey milk” (2019-2020)
  • 2. Principal Investigator: “Genetic Characterization of Marwari Horses for selection of true to breed Animals” (2015-2018)
  • 3. Principal Investigator: “Cloning, Expression and Characterization of eCG” (2010-2015)
  • 4. Co-Principal Investigator: “Gene Expression of Proteolytic System and Growth regulators in the Mammary Gland and Skeletal Muscles of Buffalo (Bubalus bubalis).” (2010-2012)
  • 5. Co-Principal Investigator: “Characterization of non-descript, geographically distinct indigenous donkeys.”(2010-2014)
  • 6. Co-Principal Investigator: “Molecular characterization of Indigenous breeds of horse for genetic diversity within and between different breeds.” (2006-2012)
  • 7. Co-Principal Investigator: “Studies on biochemical properties of donkey milk with reference to its biological role.” (2012-2015)
  • 8. Co-Principal Investigator: “Development of DNA Typing based Parentage testing in Horses.” (2015-2017)
  • 9. Co-Principal Investigator: “Development of diagnostics for sensing candidate biosignatures of buffalo reproduction.” (2016-2018)
  • 10. Co-Principal Investigator: “Assessment, evaluation and identification of physical, biochemical and genetic factors affecting stallion fertility.” (2018-2021)

DST-SERB Funded Project (December 2018-2021)

Principal Investigator: “Exploration of genomic signatures for indigenous horses using next-generation sequencing approaches”

Read more