Dr. Baldev Raj Gulati

Principal Scientist
Discipline: Veterinary Microbiology
E-mail ID : brgulati@gmail.com


 

Name                                      :           Gulati, Baldev Raj

Field of Specialization           :           Veterinary Microbiology

Present Position                     :           Principal Scientist (Veterinary Microbiology)

Educational Qualifications

Degree

University/ Institution

Year

Distinction, if any

Post-doctoral Associate

College of Veterinary Medicine, University of Minnesota, St Paul, USA

2000-01

Post-doctoral Associateship, U of M, USA

Ph.D.

CCS Haryana Agricultural University, Hisar, India

1997

Awarded CSIR Senior Research Fellowship

M.V.Sc.

CCS Haryana Agricultural University, Hisar, India

1991

Awarded HAU Merit Fellowship

B.V.Sc. & A.H. (Hons.)

Haryana Agricultural University, Hisar, India

1988

Awarded Two Gold Medals

Employment record

Position Held

Institution

Period of Appointment

From

To

Principal Scientist

National Research Centre on Equines, Hisar

January 1, 2009

Contd.

Senior Scientist

National Research Centre on Equines, Hisar

April 1, 2003

December 31, 2008

Research Interests

  1. Rotaviruses cause severe acute gastroenteritis in numerous livestock animals. Our laboratory has contributed in development of sensitive and specific diagnostics for rotavirus detection, epidemiology of equine rotavirus with particular reference prevalence in diarrhoeic foals and also characterization of rotavirus strains isolated from diarrhoeic calves, sheep & goat and foals in India.

a         A sensitive and specific sandwich enzyme-linked immunosorbent assay (ELISA) has been developed employing monoclonal antibody (mAb) raised against group A rotavirus, for detection of rotavirus infection in animals and humans.

b         Epidemiological studies revealed the nation-wide prevalence of rotavirus infection in 20% of calves and 35.4% foals below one month of age.

 c        Identification of unusual genotypes (G10, P6[1], G1, G3, G6) in diarrheic calves and foals observed in India.

  1. Japanese encephalitis (JE) is a disease of human, pigs and equines. Our efforts have contributed to assess the extent of problem among equines and pig population in India.

  a        A continuous JEV sero-prevalence since 2006 in 16 different states of India has helped in identifying the JEV hot zones in the country, for horses and pigs. This has contributed in identification of animal JEV outbreaks in Assam, Manipur, Madhya Pradesh, Delhi and Haryana.

  b       Developed assays for sensitive and specific detection of JEV in animals, including haemagglutination inhibition, serum neutralization, PRNT, RT-PCR, Real-time PCR and monocolonal antibody-based ELISA.

 c        Monoclonal antibodies against JEV and WNV have been raised and a mAb-based blocking ELISA for detection of WNV in animals has been developed.

  d        JEV has been isolated from foals exhibiting neurological signs in India. Sequence analysis of envelope gene indicated that the equine JEV isolate belonged to genotype III.

  e       The whole genome of equine JEV isolate has been sequenced. The genome contains a single open reading frame (ORF) of 10,299 nt which is flanked by 86 nt and 557 nt at 5 and 3 non translated region (NTR), respectively.

  1. Equine herpesvirus 1 (EHV1) is a significant cause of abortions in pregnant mares. Our laboratory has been working on refined vaccines and diagnostics for timely diagnosis of EHV1 infection in horses.

  a       Using Indigenous EHV1 strains an inactivated EHV1 vaccine has been developed, which is very effective in preventing abortion storms in mares.

  b      A monoclonal antibody-based blocking ELISA kit has been developed for detection of EHV1 antibodies, which was released for commercial use by the honorable DG, ICAR.

  c      A type-specific ELISA for differentiation of EHV1 and EHV4 has been developed using the recombinant expressed glycoprotein G genes of EHV-1 and EHV-4 viruses.

 d       Assays for differentiation and characterization of neuropathogenic strains of EHV1 have been developed.

 e         Assays for detection of latent EHV infections in equines        

  1. Isolation and characterization of equine mesenchymal stem cells from amniotic fluid.

Tendon injuries are common in race horses and mesenchymal stem cells (MSCs) isolated from adult and fetal tissues have been used for tendon regeneration. In the present study, we evaluated equine amniotic fluid (AF) as a source of MSCs and standardized methodology and markers for their in vitro tenogenic differentiation. Our results suggest that amniotic fluid is an accessible and effective source of mesenchymal stem cells. These cells hold the promise of value for regenerative veterinary medicine as well as for laboratory in vitro study

a        Plastic-adherent colonies were isolated from 16 of 25 AF samples by day 6 post-seeding and 70-80% cell confluency was reached by day 17.

b         These cells expressed mesenchymal surface markers (CD73, CD90 and CD105) by RT-PCR and immunocytochemistry, but did not express haematopoietic markers (CD34, CD45 and CD14).

c         In flow cytometry, the expression of CD29, CD44, CD73 and CD90 was observed in 68.83+1.27%, 93.66+1.80%, 96.96+0.44% and 93.7+1.89% of AF-MSCs, respectively.

d         Upon supplementation of MSC growth media with 50 ng/ml bone morphogenic protein-12 (BMP-12), AF-MSCs differentiated to tenocytes within 14 days. In flow cytometry, 96.7+1.90% and 80.9+6.4% of differentiated cells expressed tenomodulin and decorin in comparison to 1.6% and 3.1% in undifferentiated control cells, respectively.

Important Publications

1.      Mohanty N, Gulati BR, Kumar R, Gera S, Kumar P, Somasundaram RK, Kumar S. 2014. Immunophenotypic characterization and tenogenic differentiation of mesenchymal stromal cells isolated from equine umbilical cord blood. In Vitro Cellular & Development Biology-Animal 50:538-548 DOI 10.1007/s11626-013-9729-7.

2.      Gulati BR, Kumar R, Mohanty N, Kumar P, Somasundaram RK and Yadav PS. 2013. Bone morphogenetic protein-12 induces tenogenic differentiation of mesenchymal stem cells derived from equine amniotic fluid. Cells Tissues Organs 198(5):377-389 DOI 10.1159/000358231.

3.      Singha H, Gulati BR, Kumar P, Singh BK, Virmani N and Singh RK. 2013. Complete genome sequence analysis of Japanese encephalitis virus isolated from a horse in India. Arch. Virol 158:113-122.

4.      Mohanty N, Gulati BR, Gera S,Kumar S, Kumar R,  Kumar P and Yadav PS. 2013. Hematological and biochemical profile of equine umbilical cord blood and comparison with peripheral blood of mare and newborn foal. Indian Journal of Animal Sciences 83:1165-1168.

5.      Gulati BR, Singha H, Singh BK, Virmani N, Kumar S and Singh RK. 2012. Isolation and genetic characterization of Japanese encephalitis virus from equines in India. J Vet Sci 13:111-118.

6.      Malik YS, Sharma K, Vaid N, Chakravarti S, Chandrashekar KM, Basera SS, Singh R, Minakshi, Prasad G, Gulati BR, Bhilegaonkar KN, Pandey AB. 2012. Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types. J. Vet. Sci. 13:271-278.

7.      Gulati BR, Singha H, Singh BK, Virmani N, Khurana SK and Singh RK. 2011. Serosurveillance for Japanese encephalitis virus infection among equines in India. J Vet Sci 12:41-45.

8.      Virmani N, Bera BC, Singh BK, Shanmugasundaram K, Gulati BR Barua S, Vaid RK, Gupta AK and Singh RK. 2010. Equine influenza outbreak in India (2008-09): Virus isolation, sero-epidemiology and phylogenetic analysis of HA gene. Vet. Microbiology 143:224-237.

9.      Manuja BK, Prasad M, Gulati BR, Manuja A, Prasad G. 2010. Comparative efficacy of immunological, molecular and culture assays for detection of group A rotavirus from faecal samples of buffalo (Bubalus bubalis) calves. Trop Anim Health Prod. 42:1817-20.

10.  Gulati BR, Yadav RR & Singh BK. 2009. Epidemiological studies on equine rotavirus infection in foals of organized farms in India.  Indian J. Animal Sciences 79(1): 3-5.

11.  Singh BK, Virmani N and Gulati BR. 2009. Assessment of protective immune response of inactivated equine herpesvirus-1 vaccine in pregnant BALB/c mice. Indian J. Animal Sciences 79 (4): 345-349.

12.  Nagaleelavathi SP, Gulati BR and Garg SR. 2008. Seroprevalence of Japanese Encephalitis in Pigs in Haryana. Journal of Veterinary Public Health 6:37-39.

13.  Gulati BR, Deepa R, Singh BK and Rao CD. 2007. Diversity in Indian equine rotaviruses: Identification of G10, P6[1] and G1 type and a new VP7 genotype (G16) strains in significant numbers in diarrheic foals in India. J Clinical Microbiology 45:972-978.

14.  Gulati BR, Singh BK and Kumar D. 2006. Development of a monoclonal antibody-based sandwich ELISA for detection of equine rotavirus from diarrhoeic foals. Indian J Microbiology 46: 349-354.

15.  Gulati BR, Pandey R and Singh BK. 2006. Development of monoclonal antibodies against group A animal rotaviruses. Indian J. Biotechnology 5:37-41.

16.  Gulati BR, Malik P and Kumar R. 2005. Isolation and electropherotyping of equine rotaviruses from diarrhoeic foals in India. Indian J. Animal Sciences 75 (7): 745-748.

17.  Singh BK, Ahuja S and Gulati BR. 2004. Development of a neutralizing monoclonal antibody-based blocking ELISA for detection of equine herpes virus-1 antibodies. Vet. Res. Commun. 28: 437-446.

18.  Gulati BR, Munir S, Patnayak DP, Goyal SM and Kapur V. 2001. Detection of antibodies to US isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay. J. Clinical Microbiol. 39:2967-2970.

19.  Gulati BR, Patnayak DP, Sheikh AM, Poss, PE and Goyal SM. 2001. Protective efficacy of a high passage avian pneumovirus in turkeys. Avian Dis. 45: 593-597.

20.  Gulati BR, Cameron KT, Seal BS, Goyal SM, Halvorson DA and Njenga NK. 2000. Development of a highly sensitive and specific enzyme-linked immunosorbent assay based on recombinant matrix protein for detection of avian pneumovirus antibodies. J. Clin. Microbiol. 38:4010-4014.

21.Gulati BR, Nakagomi O, Koshimura Y, Nakagomi T and Pandey R. 1999. Relative frequencies of G and P types among rotaviruses from Indian diarrhoeic cow and buffalo calves. J. Clin. Microbiol. 37:2074-2076.