Dr. Anju Manuja

Principal Scientist
Discipline: Veterinary Medicine

E-mail ID : amanuja@rediffmail.com 




 

Area of Research Interests:

Non-methylated CpG motifs present in viral and bacterial DNA are one of the PAMPs recognized by mammalian innate immune system. TLR9 has been characterized as a receptor that can recognize unmethylated CpG motif within bacterial DNA and signaling by this receptor can trigger a pro-inflammatory cytokine response that influence both innate and adaptive immune responses. The research focus is on characterization of TLR9 in Indian breeds of horses, indigenous and exotic donkeys and immunostimulatory effects of its agonist CpG in equines.

Another area of interest is of nanomedicine. Recent advances in nanotechnology have suggested that nanoparticles may be effective in the delivery of therapeutic agents. Its better efficacy through increased absorption rate and decreased sedimentation rate may lead to the potential for reduced doses of the therapeutic agent to achieve the protective effect and reduced related side effects. The research is focused on development of targeted drug release therapeutics using nanoparticles in equine medicine and toxicological evaluation of nanoformulations. She has also worked in the areas of disease monitoring & surveillance, diagnosis and control of infectious diseases. 

Biography:

Dr. Anju Manuja, born on Dec. 18th, 1967, did her B.V.Sc & A.H. (Honors) in 1990, from College of Veterinary Sciences, Haryana Agricultural University, Hisar. She subsequently completed M.V.Sc in 1997 and Ph.D (Veterinary Medicine) in 2002 from the same college. She was awarded ICAR Merit Scholarship during B.V.Sc and AH, and ICAR junior research fellowship for doing M.V.Sc.  She did part of her Ph.D research work at Roslin Institute, Edinburgh (UK). Dr. Anju Manuja also worked as visiting scientist at Vaccine and Infectious Disease Organisation, Saskatoon (Canada) as BOYSCAST Fellow (DST, Government of India). She received Fast Track proposals for young Scientist from DST, Govt. of India year 2006. She worked as Veterinary Officer at Central Institute for Research on Buffaloes, Hisar before joining National Research Centre on Equines as Senior Scientist in August 2008.

During her professional career she is recipient of Bharat Jyoti award felicitated by IIFS, New Delhi, GADVASU women Scientist award honored by Indian Society for Veterinary Immunology and Biotechnology, Scientist Award for the best publication in the area of Immunology and Biotechnology, from Indian Society for Veterinary Immunology and Biotechnology in the Year 2009, Best Research paper award by ISBD in year 2006, Best Paper award in International conference "Current and Future scenarios in drug development and delivery" in 2012. Best oral presentation award in International Conference on Nano Science & Engineering Applications 2014.  

 Current Research Projects:

1. Synthesis, characterization and evaluation of drug loaded nano-formulations against Trypanosoma evansi in animal model 

2. Development of diagnostics for emergency preparedness and monitoring of emerging equine viral  diseases

3. Surveillance, monitoring and control of emerging and existing diseases of equines

 Patents:  Patent filed on "Nano-Drug Delivery for Quinapyramine Sulphate" (Patent Application No. 2560/DEL/2011)

 Selected Publications:

1.    Manuja A, Kumar P, Kumar R, Kumar B, Singha H, Sharma RK, Yadav SC. 2014. CpG-ODN class C-mediated immunostimulation and its potential against Trypanosoma evansi in equines. Int Immunopharmacol. 2014 Jul 24;22(2):366-370.

2.    Manuja A., Kumar S., Dilbaghi N, Bhanjana G, Chopra M, Kaur H, Kumar R, Manuja Kumar B, Singh SK, Yadav SC, 2014. Quinapyramine sulphate-loaded sodium alginate nanoparticles shows enhanced trypanocidal activity. Nanomedicine Nanomedicine. 9(11): 1625-1634 (2014).

3.    Manuja BK, Manuja A, Dahiya R., Singh S., Sharma R.C., Gahlot S.K. Diversity of interferon inducible Mx gene in horses and association of variations with susceptibility vis-a-vis resistance against equine influenza infection. Infect Genet Evol. 2014 Jul 24; 27C: 142-148.

4.    Pawan Kaur, Rajesh Thakur, Manju Barnela, Meenu Chopra, Anju Manuja and Ashok Chaudhury. 2014. Synthesis, characterization and in vitro evaluation of cytotoxicity and antimicrobial activity of chitosan-metal nanocomposites. Journal of Chemical Technology and Biotechnology. DOI: 10.1002/jctb.4383.

5.    Manuja A., Manuja, B.K. Kaushik Jyoti, H Singha 2013. Immunotherapeutic potential of CpG oligodeoxynucleotide in Veterinary species. Immunopharmacology and Immunotoxicology, 35(5):535-44.

6.    Manuja Anju, Manuja K. Balvinder, Kataria RS, Sethi RK, Singh RK. 2013. Comparative Analysis of Molecular Structure, Function and Expression of Buffalo (Bubalus bubalis) Toll-Like Receptor 9, Journal of Buffalo Science, 2013, 2: 63-71.

7.   Manuja Anju, Kumar Balvinder and Singh RK. 2012. Nanotechnology developments:Nanotechnology developments: opportunities for animal health and production Published: 2012-01-30 15:50:08 | DOI: 10.4081/nd.2012.e4

8.    Kumar Balvinder Manuja Anju, Aich Palok. 2012. Stress and its impact on farm animals. Frontiers in Bioscience E4, 1759-1767.

 

9.   Kumar S, Bhanjana G, Dilbaghi  N,  Manuja A. 2012Comparative investigation of cellular response of nanoparticles. Adv Mat Lett; 3(4): 345-349.

10.  Manuja Anju, Manuja K. Balvinder Dhingra Manupriya, Sarkar Susheel. 2012 Differential expression of toll-like receptor 9 by various immune compartments of buffalo (Bubalus bubalis). Indian Journal of Animal Sciences 82 (4): 427-429.

11.  Singh S. R., Sangwan A. K, Manuja A.,  Kadian S. K., and Nichani A. K. 2012 Heterogeneity of antigenic proteins in Indian isolates of Theileria annulata. Indian Journal of Parasitology. 26(2): 27-30

 

12.  Manuja Anju, Manuja K. Balvinder Dhingra Manupriya, Sarkar Susheel. 2012 Transcriptional expression of toll-like receptor 9 in buffalo (Bubalus bubalis) Indian Buffalo journal.

 

13.  Rana N, Raut A A, Khurana SK, Manuja A, and Saini A. 2012. Isolation and biotyping of Salmonella and Escherichia coli associated with neonatal buffalo calves. Indian Journal of Animal Sciences 82 (7): 676-678

 

14.  Yadav SC, Kumar R,. Manuja A, Goyal L, Gupta AK. 2012 Early detection of Trypanosoma evansi  infection and monitoring of antibody levels following treatment, Journal of Parasitic diseases.

15.  Manuja A, Virmani N., Kurrupusamy S, Vaid RK, Manuja BK, Kumar S and Singh BK. 2011. Rectal prolapse and enteritis in a foal. Online J. Veterinary Research. Vol. 15 (5): 462 -467

16.  Rana N, Manuja A, Raut A A, Khanna S and Mehrara K L. 2011.Leptospiral abortions in Nili-Ravi buffaloes in Punjab state of India Indian Journal of Animal Sciences 81 (2): 143 to 145.

17.  Rana N, Manuja ASaini, A.  2011. A study on parasitic prevalence in neonatal buffalo calves at an organized herd in Haryana. Haryana Veterinarian Vol. 50 pp. 95-97

18.  Manuja K Balvinder, Prasad M, Manuja Anju, Gulati BR, Prasad G. 2010. Comparative efficacy of immunological, molecular and culture assays for detection of group A rotavirus from fecal samples of buffalo calves. Tropical Animal Health and Production. DOI 10.1007/s11250-010-9642-y

19.  Nitin Virmani, B.C. Bera, B R. Gulati, Shanmugasundaram , K, B. K. Singh, R K Vaid, S Kumar, R. Kumar, P. Malik, S. K. Khurana, J. Singh, Anju Manuja, Ramesh Dedar, A. K. Gupta, S. C. Yadav, P. K. Chugh, P. S. Narwal, V. L.N. Thakur, R. Kaul, A Kanani, S. S. Rautmare and R.K. Singh (2010). Descriptive epidemiology of equine influenza in India (2008‐2009): temporal and spatial trends. Veterinaria Italiana, 46 (4), 449‐458.

20.  Manuja AnjuSonia Manchanda, B. Kumar, S. Khanna and R.K. Sethi. 2010. Evaluation of different DNA extraction methods from semen of buffalo (Bubalus bubalis) bull. Buffalo Bulletin. 29(2): 109-115.

21.  Rana N., Khanna S, Raut A., Bhardwaj S.R, Manuja A., Manuja B., Saini A., Kakkar S., Khurana  K.L and Sethi RK. 2010. Retrospective epidemiological analysis of mortality trends in neonatal and growing Murrah buffalo calves at an organized herd. Indian Journal of Animal Sciences 80 (10): 976 to 79

22.  Manuja K Balvinder, Prasad M, Manuja Anju,  Gulati BR, Prasad G. 2008.  A novel genomic constellation (G10P[3]) of group A rotavirus detected from buffalo calves in northern India. Virus Research, 138: 36 to 42

23.  Nichani Anil K, Dar Arshud M, Krieg Arthur M, Mirakhur Kuldip K,  Kaushik Radhey S., Griebel Philip J, Manuja Anju, Townsend Hugh G, Babiuk LA. Mutwiri George K. 2007. Systemic innate immune responses following intrapulmonary delivery of CpG oligodeoxynucleotides in sheep. Vet. Immunol. Immunopathol.115: 357 to 368

24.  Manuja Anju, Malhotra DV, Sikka, VK, Sangwan AK, Sharma R., Kumar B, Mehta BD, Gulati BR and Nichani AK. 2006. Isolates of Theileria annulata collected from different parts of India show phenotypic and genetic diversity. Vet. Parasitol. 147:242-252.

25.  Manuja Anju, P. Minakshi, Kumar Balvinder, Prasad G., Khanna S. and Sethi RK 2006. Molecular detection of BHV-1 genome in semen of buffalo bulls. Indian Buffalo journal. 4(1&2);8-11.

26.  Griebel Philip, Brownlie Robert, Manuja Anju, Nichani Anil, Mookherjee Neeloffer, Popowych Yurij, Mutwiri George, Hecker Rolf and Babuik Lorne. 2005. Bovine toll like receptor 9: A comparative analysis of molecular structure, function and expression.  Vet. Immunol. Immunopathol. 108: 11-16.

27.  Hodgson PD, Aich P, Manuja A., Hokamp K, Roche, F., Brinkman, F.S.L., Potter, A., Babiuk, L.A., and Griebel, P.J.  2005. Effects of Stress on Viral Bacterial Synergy in Bovine Respiratory Disease. Comparative and Functional Genomics, 6: 244-250.

28.  Dar Arshud, Munir Shirin, Viswanathan Satya, Manuja Anju, Griebel Philip, Tikoo Suresh, Potter Andrew, Kapur Vivek, and Babuik Lorne. 2005. Transcriptional analysis of avian embryonic tissues following infection with avian infectious bronchitis virus. Virus Research, 110:41 to 55

29.  Kirsty M., Manuja Anju, Russell George, Springbett Anthea, Susan C. Cragimile, Nichani Anil, Malhotra DV, Glass Elizabeth. 2004. Quantitative analysis of proinflammatory cytokine mRNA expression in Theileria annulata- infected cell lines derived from resistant and susceptible cattle. Vet. Immunol. Immunopathol. 99 (1-2): 87-98.

30.  Manuja A, Sangwan Arun, Kumar Balvinder and Nichani, AK. 2005. Immunological and Molecular diagnosis of Theileria annulata. Proc. Nat. Acad. Sci. 75 (B) Special issue, 144-159.

31.  Manuja Anju, Dixit VB, Kumar B., Khanna S. and  Sethi RK. 2005. Indigenous herbs in Veterinary Medicine. The Haryana Veterinarian. Vol.  42:  9-14.

32.  Kumar Balvinder, Manuja Anju and Kumar Arvind. 2004.  Single dilution ELISA for estimation of antibodies against Foot and Mouth Disease Virus. Online J. Veterinary Research. Vol. 8: 1-6.

33.  Manuja Anju, Malhotra DV, Sikka, VK, Sangwan AK, Kumar B and Nichani AK. 2003. Theileria annulata infected cell lines from different parts of India induce variable cytokine gene expression. Online J.Vet. Res. Vo.7:71-77.

34.  Dixit, V.B., Manuja, A, Manuja, B and Saini A. 2003. Farmers' rationale of indigenous buffalo husbandry practices: a critical analysis. Indian Buffalo J. 1:27-29.

35.  Manuja, A, Nichani, AK and Spooner Roger. 2002. Immune responses and fundamental alterations induced by Theileria annulata in the bovine host. In: Ramachandrans Fetschrift. 26: 1-12.

 

36.  Manuja, A., Nichani, A.K., Kumar, R., Sharma, R.D. and Kumar B. 2001. Single dilution ELISA using soluble piroplasm, cellular schizont and soluble schizont antigens for detection of antibodies against Theileria annulata. Vet. Res.32 (2). 165-73.

 

37.  Singh, S., Khatri, N., Manuja, A., Sharma, R.D., Malhotra, D.V. and Nichani, A.K., 2001. Impact of field vaccination with a Theileria annulata schizont cell culture vaccine on the epidemiology of tropical theileriosis. Vet.Parasitol. 101:91-100. 

38.  Manuja, A., Kumar, R., Nichani, A.K., Sharma, R.D. and Kumar, B. 2001.  Assays for studies on Theileria annulata using monoclonal antibodies. Indian. J. Vet. Res. 10: 21-27.

 

39.  Kumar M, Dhawan S, Manuja A, Dilbaghi N, Malhotra DV, Sangwan, AK, Nichani AK. 2001. Comparison of indirect immunoperoxidase test with indirect fluorescent antibody test for detection of antibody titres against Theileria annulata. J. Immunol. Immunopathol. 3(2): 36-40.

 

40.  Tuteja, F.C., Kapur, M.P. and Manuja, A. 2001. Multiple infusion of Cloxacillin sodium as dry period therapy for mastitis control in buffaloes. Vet. Pract. 2: 23-26.

 

41.  Khurana, K.L., Khanna S., Kumar, B., Manuja, Anju and Sethi R. 2001.  Efficacy of Ayurvedic antiseptic ointment against sarcoptic mange in buffalo calves. Vet. Pract.2 (1): 59-60

42.  Manuja, A., Nichani, A.K., Kumar, R., Rakha, N.K., Kumar, B. and Sharma, R.D. 2000.  Comparison of cellular schizont, soluble schizont and soluble piroplasm antigens in ELISA for detecting antibodies against Theileria annulataVet. Parasitol. 87:93-101.

43.  Dixit, V.B., Lall, D.; Manuja, A. and Chauhan, T.R 2000.  Farmers' rationale of indigenous buffalo husbandry practices in Haryana. Indian J. Dairy Sci. 53: 304-309.

44.  Manuja, A., Rakha, N.K., Kumar, R., Nichani, A.K., Sharma, R.D., Gupta, S.K. and Suri, D. 1999. A fixed cell ELISA for detecting antibodies against Theileria annulata. J. Immunol. Immunopathol. 1:41-48.

45.  Rakha, N.K; Nene, V.; Morzaria, S.P.; Skilton, R.; Manuja, A.; Sugimoto, C. and Onuma, M. 1997. Theileria annulata rp32 antigen: Identification, production and validation in immunodiagnosis. In Proceedings of Hokkaido Regional meet of animal disease control, Hokkaido, Japan, p14:20.  

46.  Khanna, S.; Khurana, K.L.; Tripathi, V.N.; Manuja, A. 1997.  Effect of EXAPAR on some parameters of reproductive efficiency in buffaloes. Ind. J. Anim. Res.; Vol. 18(1):41-43.

47.  Sikka, P.; Sethi, R.K. and Manuja, A. 1996.  Factors influencing absorption of colostral immunoglobulins in young buffalo calves. Indian J. Dairy Sci. 49(9): 546-550.

48.  Khurana, K.L.; Kumar, B.; Khanna, S.; Manuja, A. 1996. Effect of herbal galactagogue pyapro on milk yield in lactating buffaloes. Int. J. Anim.Sci. 11: 239-240.

49.  Khurana, K.L.; Manuja, A. and Kumar, B. 1994.  Efficacy of 'Charmil' gel against sarcoptic mange in buffalo calves. Indian Vet. Med. J. Vol.18, 136-139.

50.  Manuja Anju, Malhotra DV, Sikka, VK, Sangwan AK, Kumar B and Nichani AK. 2003. Theileria annulata infected cell lines from different parts of India induce variable cytokine gene expression. Online J.Vet. Res. Vo.7:71-77.